synthesis and characterization of physicochemical and immunological properties of recombinant ns3-g2 dendrimer conjugate

نویسندگان

f javadi department of biology, science and research branch, islamic azad university, tehran, iran.

p rahimi department of hepatitis and aids, pasteur institute of iran, tehran, iran.

mh modaressi department of biology, science and research branch, islamic azad university, tehran, iran.

m shafiee ardestani department of radiopharmacy, faculty of pharmacy, tehran university of medical sciences, tehran, iran.

چکیده

introduction: an effective vaccine against hcv infection is not available. the non-structural protein 3 (ns3) of the virus as an important immunogenic candidate has been utilized in various modules. nanostructured polymers have been recently used for efficient vaccine and drug delivery. the aim of the current study was the synthesis of rns3-g2 conjugate and preliminary evaluation of its immunogenicity. methods: the dendrimer was synthesized and conjugated with purified recombinant ns3 (rns3) protein. the physicochemical properties of the conjugate were evaluated by zeta potential, ft-ir spectra and confirmed by atomic force microscopy (afm). immunogenicity of the conjugate was assessed in balb/c mice. results: synthesis and conjugation of dendrimer g2 with the protein were confirmed and immunological assays showed that the conjugated form of the antigen induced higher titer of igg compared to rns3 antigen alone. conclusion: the results showed that the antigenic structure of rns3 was maintained when conjugated with the biodegradable and biocompatible g2 dendrimers and the immunogenic properties of the antigen were enhanced. therefore the new formulation may have potential as a vaccine candidate.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Synthesis and characterization of physicochemical and immunological properties of recombinant NS3-G2 dendrimer conjugate

Introduction: An effective vaccine against HCV infection is not available. The non-structural protein 3 (NS3) of the virus as an important immunogenic candidate has been utilized in various modules. Nanostructured polymers have been recently used for efficient vaccine and drug delivery. The aim of the current study was the synthesis of rNS3-G2 conjugate and preliminary evaluation of its immunog...

متن کامل

Design, Synthesis, Physicochemical and Immunological Characterization of Dendrimer-HBsAg Conjugate

Manufacturing new Hepatitis B virus vaccines, specifically by the use of nanoparticles, is of high global interest. In this paper, a new biocompatible and biodegradable structure of nano-sized hepatitis B virus’ surface antigen (HBsAg) was generated by conjugation with dendrimers, a low cost biodegradable and biocompatible polymer. The physicochemical properties of the conjugate were characteri...

متن کامل

design, synthesis, physicochemical and immunological characterization of dendrimer-hbsag conjugate

manufacturing new hepatitis b virus vaccines, specifically by the use of nanoparticles, is of high global interest. in this paper, a new biocompatible and biodegradable structure of nano-sized hepatitis b virus’ surface antigen (hbsag) was generated by conjugation with dendrimers, a low cost biodegradable and biocompatible polymer. the physicochemical properties of the conjugate were characteri...

متن کامل

synthesis and characterization of some macrocyclic schiff bases

ماکروسیکلهای شیف باز از اهمیت زیادی در شیمی آلی و دارویی برخوردار می باشند. این ماکروسیکلها با دارابودن گروه های مناسب در مکانهای مناسب می توانند فلزاتی مثل مس، نیکل و ... را در حفره های خود به دام انداخته، کمپلکسهای پایدار تولید نمایند. در این پایان نامه ابتدا یک دی آلدئید آروماتیک از گلیسیرین تهیه می شود و در مرحله بعدی واکنش با دی آمینهای آروماتیک و یا آلیفاتیک در رقتهای بسیار زیاد منجر به ت...

15 صفحه اول

extraction and characterization of allium irancum plant extract and its application in the green synthesis of silver nano particles and oxidation of thiocarbony1 compounds

سنتز سبز نانوذرات فلزی (nps) درسالهای اخیر توجه بسیارزیادی را به خود جلب کرده است. زیرا این پروتوکل کم هزینه وسازگار با محیط زیست از روش های استاندارد سنتز. در این پایان نامه ما گزارش میکنیم یک روش ساده و سازگار با محیط زیست برای سنتز نانوذرات نقره با استفاده از محلول آبی عصاره گیاه allium iranicum به عنوان یک عامل کاهش دهنده ی طبیعی. نانو ذرات نقره مشخص شد با استفاده از تکنیک های uv-visible، x...

synthesis and characterization of potentially biological active cyclometallated organoplatinum(ii) complexes

this work is presented in five parts. in the first part preparation of the starting complex [pt(c^n)cl(dmso)], 1, in which c^n = n(1),c(2?)-chelated, deprotonated 2-phenylpyridine, and dmso = dimethylsulfoxide, and its reaction with 1 equiv of the biphosphine ligands bis(diphenylphosphino)amine, dppa, or bis(diphenylphosphino)methane, dppm, to give the complex [pt(c^n)cl(dppa)], 2, or [pt(c^n)c...

15 صفحه اول

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید


عنوان ژورنال:
vaccine research

جلد ۲، شماره ۵، صفحات ۹۷-۱۰۰

کلمات کلیدی

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023